Extracellular Vesicle (EV) Quantification and Analysis with PICO
Detect, count, and analyse EVs with PICO
Read our app note on EV quantification Check out our PICO EV technology flyer Check out our EV poster presented at ISEV 2025 in ViennaExtracellular vesicle (EV) research has emerged as a rapidly growing field in life sciences, offering significant insights into cell-to-cell communication, disease mechanisms, and potential therapeutic applications. EVs play critical roles in various biological processes such as immune modulation, tumor progression, and tissue repair. Using absolute quantification PICO enables the precise counting of EVs in the biological sample without the need of an external standard. Additionally, with PICO you can directly analyse and quantify of both EV membrane proteins and EV cargo proteins.
ISEV 2025 - PICO to revolutionize EV research
We had an incredible 4 days at the ISEV Conference in Vienna! We were thrilled to welcome over 200 visitors to our booth and scientific poster, sharing the excitement around how PICO revolutionizes the EV research field.
Download our poster presented at ISEVEV Quantification Strategy #1
The PICO technology requires the binding of two different antibodies against the protein target. By using antibodies against two different EV surface markers that are both on the surface of the EVs (e.g. CD63 and CD9), you can directly count the number of intact EVs with PICO. The two labeled antibodies are used in equal concentration for the precise determination of EV count in biological samples
Buy our prelabeled CD9 and CD63 antibodiesEV Quantification Strategy #2
When using a single EV surface marker (e.g. CD63) to count intact EVs you can use a single antibody in your PICO Assay. To achieve this, during the antibody labeling the antibody stock is halved, one half is labeled with PICOglue BL Label and the other with PICOglue P8 Label. When using the the two different labeled antibody stock in equal concentration in the PICO assay you can determine the precise concentration of intact CD63+ EVs.
EV Cargo Quantification
PICO also enables the absolute quantification of EV cargos including proteins, protein interactions and post-translational modifications. All you need is the lysis of your EVs and two different antibodies binding simultaneously to your target.
Quantification of EVs with PICO - Application Note
In this application note we detected and quantified extracellular vesicles (EVs) from serum or cell supernatant using PICO technology. The EVs were measured with two different detection strategies: 1) using a CD63 antibody, halved and labeled with two different PICOglue Labels, we quantified CD63+ EVs, while 2) using CD9 and CD63 antibodies, we quantified CD9/CD63+ EVs.
Download the App Note