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Protein Interaction Coupling Technology

Actome's propiarety Protein Interaction Coupling Technology, or short PICO, allows you to detect and quantify proteins, protein interactions and post-translational modifications with ultra-high sensitivity. With our kits you are able to label antibodies, that you are free to choose, conduct a PICO assay and analyze the results with our free, online based AMULATOR software. Read our PICO Handbook for an comprehensive overview.

The unique features of PICO are:

  • Ultra-high sensitivity (upper femtomolar with ordinary antibodies)
  • Zero background
  • Small sample input (down to 0.5 µL or a couple of 100s of cells)
  • Absolute quantification (quantifying molar amounts with no external standard)
  • Easy multiplexing (up to four antibodies)
  • Digital assay (counting molecules)
  • Homogeneous (measuring in non-denaturing conditions without washing steps)

Watch our technology explainer video first to learn about the PICO principle:

The PICO Principle

The PICO Workflow

What can be a PICO target?

By using a 2-plex setup (two labeled antibodies) you are able to detect and quantify:

  • I. Single proteins (by using two antibodies targeting different accessible epitopes of the same protein)
  • II. Post-translational modification (by using one antibody targeting the protein and one antibody targeting the PTM)
  • III. Protein interactions (by using two antibodies, each targeting one of the interacting proteins)
  • IV. Combinations of all of the above (e.g. protein interaction upon PTM)

By using a 4-plex setup (four labeled antibodies) you are able to detect and quantify simultaneously:

  • I. Two individual singe proteins (by using two antibodies per protein)
  • II. Two individual interactions (by using two antibodies per each interaction)
  • III. One interaction and one single protein (by using two antibodies for the interaction and two antibodies targeting the single protein)
  • IV. 'Quadratic measurement' - allowing to quantify an interaction and both interaction partners (by using two antibodies for each interacting protein)
  • V. 'Triangular measurement' - e.g. quantify the total amount of a protein and simultaneously quantifying the ratio of a PTM (by using three antibodies for one target)