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splitPICO

The splitPICO workflow is an advanced version of the PICO technology enabling the detection and quantification of up to 12 different protein targets in a single PICO Assay. splitPICO is the best option for monitoring the activity of entire signaling pathways in one experiment. 

Interested? Contact us and become a splitPICO pioneer. 

The splitPICO workflow

With the innovative splitPICO workflow, we have significantly expanded the detection capacity, enabling the analysis of twice (or more) as many targets without additional sample requirements. This is achieved by introducing a new set of labels (PICOglue Beta Labels), which have distinct primer binding sites compared to the ‘original’ labels (PICOglue Alpha Labels) but the same PICO Probe binding site. 

 

In the splitPICO workflow, the sample is first mixed and incubated with a panel of up to eight labeled antibodies: four antibodies labeled with distinct Alpha Labels and four with distinct Beta Labels. After incubation, the mixture is split into two master mixes (hence the name), one containing primers specific for the Alpha Labels and the other for the Beta Labels. During amplification the Alpha Primers will allow only the amplification of Alpha Labels while Beta Primers allow the amplification of Beta Labels.

splitPICO - application note

In this app note we describe the basics of splitPICO and demonstrate its capabilities in measuring proteins (HER2; HER3; S6K1), protein interactions (HER2::HER3), and post-translational modifications (p4EBP1) in the same signaling pathway.

Download the app note