Detect and quantify single proteins from biological samples with PICO
Protein analysis is crucial for understanding the biological processes in the human body, as proteins play a fundamental role in various functions, including tissue building, metabolism regulation, and immune response. Absolute quantification, which measures the exact amount of a protein in a sample, is vital for accurate comparison between differen samples and for detecting even small changes in protein levels, especially in disease biomarkers. Therefore, protein analysis with absolute quantification is a critical tool in research and clinical settings.
Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on a single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning
The confirmation of the CRISPR/Cas9 mediated knockout of RANKL in mesenchymal stem cell clones was achieved with PICO. With PICO we could distinguish between homozygous and heterozygous knock-outs from as little as 1,000 cells. Click here to get free access to the full publication.
Please note: In this paper PICO was called Emulsion Coupling (EMUC) and the read-out was performed with the Bio-Rad dPCR instrument. Since January 2022 PICO works exclusively on QIAGEN's QIAcuity Digital PCR System.