Protein Quantification with PICO

Detect and quantify single proteins from biological samples with PICO

Read our publication in PLOS One Read our app note about HER2 quantification

Protein analysis and quantification is crucial for understanding the biological processes in the human body, as proteins play a fundamental role in various functions, including tissue building, metabolism regulation, and immune response. Absolute quantification, which measures the exact amount of a protein in a sample, is vital for accurate comparison between differen samples and for detecting even small changes in protein levels, especially in disease biomarkers. Therefore, protein analysis with absolute quantification is a critical tool in research and clinical settings.

Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on a single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning

The confirmation of the CRISPR/Cas9 mediated knockout of RANKL in mesenchymal stem cell clones was achieved with PICO. With PICO we could distinguish between homozygous and heterozygous knock-outs from as little as 1,000 cells. Click here to get free access to the full publication.

Please note: In this paper PICO was called Emulsion Coupling (EMUC).