Frequently Asked Questions (FAQ)
Find answers to common questions about our PICO Kits and Services. Need more help? Contact Actome's customer support team.
What is PICO?
Protein Interaction Coupling (PICO) is an immunoassay for the detection and absolute quantification of Extracellular Vesicles, Proteins, Protein Interactions, and post-Translational Modifications. PICO combines the advantages of immunoassays with digital PCR technology, by translating protein status into DNA barcodes.
What can PICO measure?
With PICO you can measure EVs, proteins, protein interactions, and post-translational modifications from a soluble biological sample.
What are the advantages of PICO compared to other protein detection assays?
PICO outperforms traditional methods like Western blot, coIP, ELISA, and PLA by offering absolute quantification, zero background, low sample input, and multiplex and high-throughput capability.
Where can I purchase the PICO kits?
The PICO kits are available for purchase in Actome’s webshop.
Technical questions
Find answers to common technical questions below. For further inquiries, refer to our FAQ or contact our support team at support@actome.de.
PICO Assay
The kit contains materials to perform 120 dPCR reactions in 24-well dPCR plates or 480 dPCR reactions in 96-well dPCR plates.
Use 1-2 µg of lysed cell material for high-expressing targets and around 10 µg for low-expressing targets.
PICO can analyze any liquid sample, including supernatants and blood serum. Samples must be liquefied, lysed, and homogenized per standard lab protocols.
Data Analysis
Use AMULATOR for analyzing your PICO data.
The data analysis is completely free with no additional costs.