Application Notes
Explore the latest insights from the PICO technology.
Quantification of protein-protein interactions (PPI) with PICO
In this application note we detected and quantified HER2 and HER3 in addition to their interaction using PICO technology. Using Absolute Quantification, we could show that PICO is approximately ~200 fold more sensitive than co-immunoprecipitation (coIP).
Therefore, PICO is a powerful technology to quantify protein interactions and thus to monitor the activity of signaling pathways.
Quantification of post-translational modification (PTM) with PICO
In this application note we detected and quantified 4EBP1 and its phosphorylated form using PICO technology. Using Absolute Quantification, we could show that upon treatment of BT474 or MCF7 cells with the kinase inhibitor Dactolisib, the phosphorylation of 4EBP1 is decreased ~200 fold.
Therefore, PICO is a powerful technology to quantify post-translational modifications and to monitor the activity of signaling pathways.
Quantification of extracellular vesicles (EVs) with PICO
In this application note we detected and quantified extracellular vesicles (EVs) from serum or cell supernatant using PICO technology. The EVs were measured with two different detection strategies: 1) using a CD63 antibody, halved and labeled with two different PICOglue Labels, we quantified CD63+ EVs, while 2) using CD63 and CD9 antibodies, we quantified CD9/CD63+ EVs. These findings highlight the versatility of PICO in sensitive and accurate detection of various protein targets.
splitPICO - an advanced PICO workflow for simultaneous detection of up to 12 targets
The splitPICO workflow is an upgrade to the canonical PICO workflow enabling the simultaneous detection of 12 different protein targets in a single PICO experiment. In this app note we describe the basics of splitPICO and demonstrates its capabilities in measering proteins, protein interactions, and post-translational modifications in the same signaling pathway.
Comparing the Sensitivity of PICO vs. Western Blot
In this application note we compared the sensitivity of PICO to western blot. We chose to detect the breast cancer biomarker HER2/ErbB2 in two different breast cancer cell lines, BT474 and MCF7. Through our analysis, we found that PICO demonstrated approximately 200-fold higher sensitivity compared to western blot. These findings further support the advantages of using PICO for sensitive and accurate protein detection.
Absolute vs. Relative Quantification with PICO
In this application note we compared the two PICO quantification methods: the Absolute Quantification and the Relative Quantification. Using HER2 as the model protein we showed that BT474 cells have approximately 120-fold more HER2 than MCF7 cells.