PIQuant is PICO BioScience's free online data analysis software to evaluate PICO Assays
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Upon purchasing a PICO Kit, you will receive a Token granting you access to PIQuant. This access is valid for a period of six months. Should you require assistance with the usage of the software or if your token expires before you have completed all necessary analyses, don't hesitate to get in touch with customer support.
PIQuant is a software intended to guide users on how to obtain absolute quantitative data from a PICO experiment.
Key capabilities and possibilities using PIQuant:
- PICO Experiment Data Analysis: The primary purpose is to analyze data from a Protein Interaction Coupling (PICO) immunoassay experiment, which is used for the characterization and quantification of Extracellular Vesicles (EVs), proteins, protein interactions, and post-translational modifications.
- Quantification Options: It offers two main evaluation options for PICO data:
- Relative Quantification (RQ): Uses an external standard or control sample for internal reference.
- Absolute Quantification (AQ): Analysis performed without an external standard.
- Data Processing: It allows users to select content to analyze (antibody mix, antibody pairs, samples) and how the couplex data will be processed. Recommended processing for maximal accuracy of Absolute Quantitative data includes:
- Lambda normalization, Antibody Binding Control (ABC), and Labeling Efficiency (LE) corrections (Couplexes LN-ABC-LE).
- Note: The labeling efficiency correction should not be applied to Extracellular Vesicle (EV) samples.
- Quality Control: It features a Quality Control tab that details performance features such as the number of valid partitions, positive non-template control (NTC) partitions, average lambda, and ABC values, with acceptable ranges suggested by PIQuant.
- Evaluation and Results: The Evaluation tab displays experimental results, including:
- Couplex graphs that give a first insight into the relative abundance of the target.
- The ability to run absolute quantification after indicating antibody concentration and target lambda (e.g., 500 pM and 0.15 for regular AQ).
- The option to select only data from the "high-side" or "low-side" for absolute quantification, which is necessary due to the "hook effect" that can occur in immunoassays.
- Data Download: Users can download the analysis data and graphs once the analysis is completed.
- Device Support: PIQuant currently supports dPCR devices from Qiagen and Bio-Rad (beta).